HCP Kit ELISA

N° du produit ABIN7764585

Ce kit ELISA E. coli HCP est un kit ELISA Colorimetric conçu pour quantifier E. coli HCP.

Aperçu rapide pour HCP Kit ELISA (ABIN7764585)

Antigène: HCP (Host Cell Proteins (HCP))

Reactivité: E. coli

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.39 ng/mL - 100 ng/mL

Application: ELISA

Type d'échantillon: Biological Therapeutics, Cell Culture Supernatant, Purified Protein

Détail du produit HCP Kit ELISA

Seuil minimal de détection: 0.39 ng/mL

Fonction: ELISA Kit for the quantitative Measurement of host cell protein (HCP) residues in biopharmaceuticals by E. coli (Bl21(DE3), TG1, W3110) expression systems

Analytical Method: Quantitative

Réactivité croisée (Details): No cross-reaction exists between the homologous antigens.

Attributs du produit: E. coli Host Cell Protein (E. coli HCP) ELISA kit, G3 (Bl21(DE3), TG1, W3110)

Ingrédients:

• 1. Pre-Coated Microplate (Detachable)
• 2. Standard (Stock Solution) -500µg/ml
• 3. Detection antibody (100x)
• 4. TMB Substrates
• 5. Stop Solution
• 6. Wash Solution (100x)
• 7. Diluent Buffer (10x)
• 8. Plate Sealer
• 9. Instruction Manual

Matériel non inclus:

• 10-1000μL pipettor
• Multichannel pipettor
• 1L sterilized deionized water or ultrarpure water
• Sterilized EP tubes
• Absorbent Paper
• Microplate reader
• High-speed centrifuge
• Mini Centrifuge
• Microplate washer or washing bottles
• Data analysis and graphing software

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 3.5 h

Plaque: Pre-coated

Protocole: This ELISA kit is applied to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microplate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by in putting the ODvalues of the samples into the standard curve equation, calculate the concentration of the target protein in the sample.

Précision du teste: Intra-assay precision: 7.3-13.1 %
Inter-assay precision: 8.1-11.5 %

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: HCP (Host Cell Proteins (HCP))

Autre désignation: Host Cell Proteins